Coronavirus COVID-19

2020 action plan

    COVID-19 Complete Workflow

    • Collection & Preservation
    • RNA Isolation
    • PCR Based Detection
  • + PCR Components

We are ready to do our part.


SAMPLE COLLECTION & PRESERVATION

Swab Collection and Total Nucleic Acid Preservation System (Cat. 68800)

  • No need to immediately process samples
  • Preserve total DNA, including viral DNA, at room temperature 2 years
  • Preserve total RNA, including viral RNA, at room temperature for 2 months
  • Inactivate microorganisms and viruses for safe and easy transport and handling
  • Compatible with most DNA and RNA isolation methods
  • Compatible with many sample types, including nose, mouth, throat and skin
  • Validated for COVID-19 detection

Preserve total RNA including viral RNA with Norgen’s convenient all-in-one collection system for up to 2 months *

fig-1_0

Figure 1. Isolation of high quality RNA from HeLa cells stored in Norgens Total Nucleic Acid Preservative Tube at room temperature for up to 30 days. HeLa cells were stored in Norgens Total Nucleic Acid Preservative Tube (DR) and RNAlater (RL) for 30 days, and the RNA was isolated from the preserved samples using both an ethanol precipitation method and Norgens Total RNA Purification Kit (Cat. 17200). As it can be seen, the EtOH precipitation method failed to isolate RNA from the RNAlater preserved sample. High quality RNA was isolated from the samples preserved in Norgens Total Nucleic Acid Preservation Tubes using both isolation methods, indicating excellent preservation of the RNA and compatibility with various isolation methods.


RNA ISOLATION

Total RNA Purification Kit (Cat. 17200, 37500, 17250)

17200-web
  • Extract high quality & purity total RNA including miRNA
  • No phenol step required
  • Bind & elute all RNA irrespective of size or GC content, without bias
  • Efficiently extract small RNA irrespective of GC content
  • Very sensitive & linear down to a few cells without the need for carrier RNA
  • Convenient & fast spin column format
  • Isolate from a wide variety of specimens
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services

For rapid purification of total RNA, including microRNA, without phenol

fig-1_0

Figure 1. High Quality of Isolated RNA with Complete Size Range. Unlike most competitors' kits, Norgen's Total RNA Purification Kit allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA, without the use of phenol. Total RNA was isolated from 1 x 109 E. coli cells using Norgens Total RNA Purification Kit and a competitors kit. Five microliters and 1 µL of the 50 µL isolated RNA was analyzed on an agarose gel (Panel A) and the Agilent® 2100 BioAnalyzer RNA Nano 6000 chip (Panel B), respectively. Note the presence of small RNA species (red square) in the samples isolated via Norgen's kit and the absence of these RNA species in the competitor RNA preparation.


PCR BASED DETECTION

2019-nCoV TaqMan RT-PCR Kit (Cat. TM67100)

  • Detection kit for SARS-CoV-2, which causes COVID-19 (2019-nCoV)
  • Ready to use format containing everything required for detection
  • Available in 1 format for analysis:
  • For our COVID-19 information brochure click here

fig-1_0

Figure 1. Analytical sensitivity for the detection of 2 SARS-CoV-2 nucleocapsid target genes and the RNase P internal Control target as recommended by the Centers for Disease Control and Prevention (CDC). Panel A represents the 2019-nCoV_N1 target, Panel B represents the 2019-nCoV_N2 target and Panel C represent the RNase P internal Control target. The analytical sensitivity of Norgens 2019-nCoV TaqMan RT-PCR Kit was determined by analyzing a dilution series of quantified Norgens 2019-nCoV RT-PCR Positive Control (Cat. PC67102) in triplicate (n=3) based on the use of TaqMan® technology. The linear range of Norgens 2019-nCoV TaqMan RT-PCR Kit has been determined to detect as low as 2 copies/µL with a confidence ≥ 90%.


PCR COMPONENTS

2019-nCoV Primer & Probe Mixes

  • Primer & Probe sets for SARS-CoV-2, which causes COVID-19 (2019-nCoV)
  • Contains all 3 CDC developed assays in individual tubes
  • Each assay is targeting one of the 2 nucleocapsid target genes RNA (N1, N2) and an RNase P for internal control

Ordering Information

Cat. TM67101 50 Rxns

2019-nCoV RT-PCR Positive Control

PositiveControl(Cat.PC67102)-TheoreticalCopyNumber

2019-nCoV_N1F 2019-nCoV_N2F RNAse P-F
2x108 + + +
2x107 + + +
2x106 + + +
2x105 + + +
2x104 + + +
2x103 + + +
2x102 + + +
2x101 + + +
NTC N/D N/D N/D
  • Positive control for SARS-CoV-2, which causes COVID-19 (2019-nCoV)
  • Contains 2 nCoV nucleocapsid target gene RNA (N1, N2) and RNase P.
  • Compatible with CDC primer/probe set

Ordering Information

Cat.PC67102 50 µL

2X One-Step RT-PCR Master Mix

  • Convenient ready-to-use solution
  • High sensitivity and yield
  • Robust amplifi cation

Ordering Information

Cat. 28113 100 Rxns
Cat. 28114 200 Rxns
Cat. 28115 500 Rxns
17200-web
17200-web

Figure 1. Performance of Norgens 2X One-Step RT-PCR Master Mix (28113) to detect SARS-CoV-2 using Norgens 2019-nCoV Primer & Probe Mixes (TM67101) and Norgens 2019-nCoV RT-PCR Positive Control (PC67102). The one-step RT-PCR reaction was prepared according to the CDC protocol and the sensitivity of detection of the three targets; 2019-nCoV_ N1 (Circle), 2019-nCoV_ N2 (Triangle) and RNase P (Cross), was compared with Competitor Ns product (Red).

//paste this code under the head tag or in a separate js file. // Wait for window load $(window).load(function() { // Animate loader off screen $(".se-pre-con").fadeOut("slow");; });